Journal: bioRxiv
Article Title: Simultaneous Inhibition of ACLY and OGDH Has a Synergistic Effect on Hepatocellular Carcinoma Cell Lines
doi: 10.64898/2026.04.19.716936
Figure Lengend Snippet: Cell viability assays were conducted using the WST-1 method to evaluate the effects of valproic acid (VPA) and bempedoic acid (BA), alone or in combination, on the viability of THLE-2, Hep3B, and Huh7 cells. (A) The outlines of the WST-1 assay protocol were followed in the study. The effects of (B) valproic acid (0,1, and 2mM), (C) bempedoic acid (0,10, and 25μM), and (D) their combinations on THLE-2 cells, respectively. Similarly, (E–G) show the effects on Hep3B cells and (H–J) present corresponding data for Huh7 cells. Cell viability was measured at 72 and 96 hours post-treatment, as shown at the top of each plot. Red-highlighted values indicate fold change inhibitory effects compared to the control, where values less than one indicate inhibition and values greater than one represent stimulation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns-non-significant. Analysis was performed using one-way ANOVA. Data are shown as mean ± SD.
Article Snippet: The immortalized human liver epithelial cell line THLE-2 and human HCC cell lines Hep3B were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).
Techniques: WST-1 Assay, Control, Inhibition